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<H2>Cell Respiration . . .</H2>
<P>... <B>check your knowledge in (fairly) easy stages.</P>
<P><A name=3DTOP>Try the following</A> in order:</P>
<OL></B>
  <LI><A =
href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#R1"><B>Basics about =

  cell respiration</B></A><B>=20
  <LI><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#R2">Going =
deeper=20
  with glycolysis </A>
  <LI><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#R3">The =
Krebs=20
  Cycle</A>=20
  <LI><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#R4">The =
Electron=20
  Transfer Chain</A> </LI></OL></B>
<P>Marks are shown in brackets. The marking point in the answers are =
separated=20
by ; . The / denotes an acceptable alternative wording. If unsure about =
anything=20
and you can't work it out from your textbook <A=20
href=3D"mailto:biodesk@yahoo.com">email me!</A></P><B>
<P><A name=3DR1>Respiration 1</A> - the basics.</P>
<OL></B>
  <LI>What <I>is</I> cell or tissue respiration? (2 )=20
  <LI>What other way is the term 'respiration' commonly understood? (1)=20
  <LI>What is the connection between your answers to 1 and 2? (2)=20
  <LI>Cell respiration results in energy being stored in a short term =
chemical=20
  substance called? (1)=20
  <LI>What is the substance you have named in 4 synthesised from or =
converted=20
  to? (1)=20
  <LI>Give an overall <U>word</U> equation for aerobic cell respiration. =
(1)=20
  <LI>Name the FOUR key stages of cell respiration. (4)=20
  <LI>What are the useful end products of the first stage? (2)=20
  <LI>How much of these products is produced? (2)=20
  <LI>What name is given to respiration when oxygen is absent? (1)=20
  <LI>What end products are possible from this type of respiration a)in =
animals=20
  and b) yeast? (2)=20
  <LI>What name is given to respiration when oxygen is present?=20
(1)</LI></OL><B><I>
<P>Now check the answers and add up your score:</P>
<UL></I>
  <LI>20 Excellent! Now try Respiration 2 quiz.=20
  <LI>15-19 Not bad- but brush up on your weak areas before proceeding.=20
  <LI>14 or less Go back over the test as many times as it takes to get =
you up=20
  to 20! Writing the answers down will be a definite aid to your =
learning at=20
  this stage, rather than just rehearsing the answers =
mentally.</LI></UL></B>
<P>&nbsp;</P>
<P>
<HR>

<P></P><B>
<P>Answers to Respiration 1:</P>
<OL></B>
  <LI>The breakdown/oxidation of glucose to release energy;=20
  <P><BR>It may occur in the presence or absence of oxygen;</P>
  <LI>Breathing- commonly called (whole) body respiration;=20
  <LI>Body respiration supplies the oxygen;=20
  <P>and removes the carbon dioxide; used in cell respiration</P>
  <LI>Adenosine Triphosphate (ATP will usually be OK!);=20
  <LI>Adenosine Diphosphate (ADP);=20
  <LI>GLUCOSE + OXYGEN <FONT face=3DWingdings>=E0</FONT> ENERGY + WATER =
+ CARBON=20
  DIOXIDE (if you said ATP instead of energy, that is fine!);=20
  <LI>Glycolysis; Link Reaction; Krebs/TCA/Citric acid Cycle; Electron =
Transfer=20
  Chain/Oxidative phosphorylation;=20
  <LI>Pyruvate/Pyruvic Acid; (both are usually acceptable) ATP;=20
  <LI>2 pyruvates; 4 ATP's but 2 ATP's simply repay that used to start =
the whole=20
  thing rolling, making a NET product of <U>2</U> ATP's;=20
  <LI>Anaerobic respiration;=20
  <LI>Animals- Lactic Acid/Lactate; Yeast- Alcohol/Ethanol; (NOT =
ethan<U>al</U>)=20
  which is an intermediate substance).=20
  <LI>Aerobic Respiration;</LI></OL>
<P><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#TOP">TOP OF=20
PAGE</A></P>
<P><A href=3D"http://uk.geocities.com/biodesk/AL_index.htm">Back to A =
Level Index=20
Page</A>=20
<P>
<HR>

<P></P>
<P><B><A name=3DR2>Respiration 2 </A>- Going deeper with =
glycolysis</B></P>
<OL>
  <LI>Remind me- what is produced (and how much) at the end of =
glycolysis? (3)=20
  <LI>How many carbons are there in the original glucose molecule? (1)=20
  <LI>How is the substance triose phosphate produced during =
glycolysis?(1)=20
  <LI>What is another name (and abbreviation) for triose phosphate? (2)=20
  <LI>Triose phosphate is then 'oxidised' to form another 3-carbon =
substance=20
  before we get to pyruvate.(for A level we only need to note one-though =
there=20
  are several!) Name the substance and give its abbreviation. (2)=20
  <LI>What gaseous substance is produced by the oxidation of GALP? (1)=20
  <LI>Name the substance that 'mops up' your answer to 6. Which vitamin =
is vital=20
  for this substance to be present?(2)=20
  <LI>Name the process whereby the glucose molecule's activation energy =
is=20
  overcome. (1)=20
  <LI>What does your answer to 9 actually mean in chemical terms? (2)=20
  <LI>When during glycolysis, is ATP formed? (2)=20
  <LI>Where in the cell does glycolysis happen? (1)=20
  <LI>How much ATP do you think there is in the human body- 1g, 5g, 50g, =
500g,=20
  1kg? What are the implications of this? (2)</LI></OL><B><I>
<P>Now check your answers </P></B></I>
<P>20- excellent. Carry on to the next quiz-'<B>Going deeper with=20
Hans'</P></B>19- recheck your weak areas before carrying on to the next =
quiz.=20
<P>Below 15 - keep going over the questions, writing your answers down, =
until=20
you get 20!</P>
<P>&nbsp;</P>
<P>&nbsp;</P><B>
<P>Answers to 'Going deeper with glycolysis'</P>
<OL></B>
  <LI>2 molecules of ATP (NET); and 2 molecules of pyruvate; from one =
molecule=20
  of glucose;=20
  <LI>Six - it is shown as 6C;=20
  <LI>By splitting the original 6C glucose into two 3 carbon molecules =
of triose=20
  phosphate;=20
  <LI>Glyceraldehyde-3-phosphate; or GALP; (note the '3-phosphate' part =
of GALP=20
  is where the name 'triose phosphate' comes from. In other words it has =
3 (tri)=20
  phosphates.)=20
  <LI>Glycerate-3-phosphate; GP;=20
  <LI>Hydrogen;=20
  <LI>NAD<SUP>+</SUP> (becomes NADH); NAD (nicotinamide adenine =
dinucleotide) is=20
  derived from the B vitamin <B>nicotinic acid</B>; check if you need to =
know=20
  about this vitamin in your human biology units-remember synoptic =
questions!=20
  <LI>Phosphorylation;=20
  <LI>A phosphate molecule is added to the glucose molecule; it is =
derived from=20
  ATP; (which now becomes ADP due to the loss of one phosphate)=20
  <LI>During the conversion of GALP to GP; and during the conversion of =
GP to=20
  pyruvate;=20
  <LI>Cytosol; (you may get away with 'cytoplasm'-though this really =
refers to=20
  everything, including organelles, that is between the nucleus and cell =
surface=20
  membrane).=20
  <LI>Around 50g;. You may not be directly asked this, but it follows =
that as we=20
  use about 140kg of ATP daily there must be an incredible rate of =
turnover,=20
  breakdown and regeneration! ATP really is a 'short-term energy store'. =
It's an=20
  important principle to remember.</LI></OL>
<P><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#TOP">TOP OF=20
PAGE</A></P>
<P><A href=3D"http://uk.geocities.com/biodesk/AL_index.htm">Back to A =
Level Index=20
Page</A>=20
<P>
<HR>

<P></P>
<P>&nbsp;</P>
<P>&nbsp;</P><B>
<P><A name=3DR3>Respiration 3</A> - Going deeper with Hans.</P></B>
<OL></B>
  <LI>During aerobic respiration, where does the pyruvate go after =
glycolysis?=20
  (1)=20
  <LI>What substance does it combine with? (1)=20
  <LI>When the pyruvate combines with the substance named in answer 2; =
what two=20
  substances are produced? (2)=20
  <LI>"acetyl CoA combines with .............. .............. to form=20
  .............. .............. "(2)=20
  <LI>Which two substances 'mop up' the hydrogen produced during the =
Krebs=20
  Cycle? (1) <U>
  <LI>Precisely</U> where in the cell does the Krebs Cycle take =
place?(1)=20
  <LI>The hydrogen atoms are the ................ power for the ETC (1)=20
  <LI>Where is most of the ATP produced (precisely) during respiration?=20
(1)</LI></OL><B><I>
<P>Now check your answers </P></B></I>
<P>10- excellent. Carry on to the next quiz-"<B>Electron =
Transfer"</P></B>
<P>6-9 recheck your weak areas before carrying on to the next quiz.</P>
<P>5 or less - keep going over the questions, writing your answers down, =
until=20
you get 10!</P>
<P>&nbsp;</P><B>
<P>Answers to Respiration 3.</P></B>
<OL>
  <LI>It enters the mitochondria;=20
  <LI>Acetyl co-enzyme A ( or acetyl CoA);=20
  <LI>Carbon dioxide; and hydrogen;=20
  <LI>"acetyl CoA combines with oxaloacetic acid to form citric acid "=20
  <LI>NAD and FAD;=20
  <LI>Matrix of the mitochondria;=20
  <LI>Reducing;=20
  <LI>On the stalked particles;</LI></OL>
<P><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#TOP">TOP OF=20
PAGE</A></P>
<P><A href=3D"http://uk.geocities.com/biodesk/AL_index.htm">Back to A =
Level Index=20
Page</A>
<P>
<HR>

<P>&nbsp;</P><B>
<P><A name=3DR4>Respiration 4</A> - Electron Transfer Chain</P></B>
<P>Fill in the blanks.</P>
<P>The ........................ attached to NAD and =
........................ is=20
transferred to a number of ........................ containing=20
........................ carriers called ......................... In =
the=20
process the atoms of ........................ are split into protons and =

......................... The former are not passed along the carriers =
but=20
eventually recombine with ........................ to form=20
.........................</P>
<P>The negatively charged ........................ however ARE passed =
from=20
carrier to carrier, alternately ........................ and=20
........................ them.</P>
<P>As this happens, energy is released at each stage and is used to =
power=20
........................ pumping. This process works as follows.</P>
<P>The energy released at each stage of the transfer chain is used to =
pump=20
........................ across the ........................=20
........................ into the ........................=20
......................... This creates a pH gradient as well as a=20
........................ difference. The result is that =
........................=20
diffuse back into the mitochondrion (probably via the =
........................=20
particles).</P>
<P>As this diffusion occurs, the enzyme ........................=20
........................ is activated resulting in the formation of the=20
substance ........................</P><B><I>
<P>Now check your answers</P></B></I>
<P>You need to score 22!</P><B>
<P>&nbsp;</P>
<P>&nbsp;</P>
<P>Answers</P></B>
<P>The HYDROGEN attached to NAD and FAD is transferred to a number of =
IRON=20
containing PROTEIN carriers called CYTOCHROMES. In the process the atoms =
of=20
HYDROGEN are split into protons and ELECTRONS. The former are not passed =
along=20
the carriers but eventually recombine with OXYGEN to form WATER.</P>
<P>The negatively charged ELECTRONS however <I>are </I>passed from =
carrier to=20
carrier, alternately OXIDISING and REDUCING them.</P>
<P>As this happens, energy is released at each stage and is used to =
power PROTON=20
pumping. This process works as follows.</P>
<P>The energy released at each stage of the transfer chain is used to =
pump=20
PROTONS across the INNER MEMBRANE into the INTERMEMBRANE SPACE. This =
creates a=20
pH gradient as well as a POTENTIAL difference. The result is that =
PROTONS=20
diffuse back into the mitochondrion (via the STALKED particles).</P>
<P>As this diffusion occurs, the enzyme ATP SYNTHETASE is activated =
resulting in=20
the formation of the substance ATP.</P>
<P><A href=3D"http://uk.geocities.com/biodesk/bioresp1.htm#TOP">TOP OF=20
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